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C4—A/A31 HPLC Guard Columns from UHPLCS Scientific Instruments
What is a guard column?
A guard column is a protective column or cartridge installed between the injector and the analytical column. It serves to remove the impurities and suspended solids from reaching the analytical column.
Desirable features of guard columns
- Guard column should have preferably the same packing as the analytical column to eliminate separation complications
- Internal ID of guard column should be comparable to analytical column to minimize back-pressure. Shorter guard column length is preferable but it should be long enough to prevent strongly retained compounds from reaching the main column
- Frit facing the injector should be removable for cleaning by removal of about 2 mm of material and filling with fresh material
