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Precautions for the use of liquid chromatographic columns:
1) pH range of the column. C18 reversed-phase columns are generally suitable for samples in the pH range of 2~8, so the pH of the buffer must be adjusted precisely. The buffer's pH value and the sample's pH value must be adjusted precisely, and the concentration of the buffer must not exceed 0.02 mol/L.

2) Pressure range of the column. Under normal conditions, the flow rate will gradually increase, and the column pressure will gradually increase until it maintains a more stable range. The maximum pressure should not exceed 4000 Psi, and the column pressure should be recorded every day according to the instruction of each column.

3) Flow rate range of the column. Set the flow rate according to the different columns.

4) The column direction should not be reversed (otherwise, the stationary phase will be damaged).

5) When using buffer, rinse the pipeline and column with ultrapure water for 1 hour immediately after sample preparation. Then, rinse with methanol for 40 minutes or more to ensure that the entire pipeline and column are clean. Then rinse with methanol for more than 40 minutes to ensure that the whole pipeline and column are kept in the organic solvent.

(6) If the instrument is not used for a long time, the column should be taken off and sealed with a plug. Note that the column should not be stored in pure water but in the organic phase (such as methanol, etc.) because pure water is easy to grow mould.