Liquid chromatographic HPLC column filler Silica gel packing

Silica gel is the most widely used substrate for Liquid Chromatography stationary phases. Its physical and chemical properties make it suitable for a wide variety of analytical and preparative separations.

Silica gel also provides outstanding characteristics regarding selectivity, capacity, and efficiency for all types of chromatography. Spherical silica gels consist of rigid beads, manufactured by dry or wet processes, capable of withstanding high flow rates and high pressure.

Silica gel is the most prevalent matrix in HPLC fillers. In addition to its high strength, it provides a surface to which various ligands can be bonded by sophisticated silylation techniques to do packings for reversed-phase, ion exchange, hydrophobic interaction, hydrophilic interaction, or molecular exclusion chromatography. Silane-based fillers are suitable for a wide range of polar and non-polar solvents. The disadvantage is that they are unstable in alkaline water-soluble mobile phases. Typically, the recommended routine analytical pH range for silica-based packings is 2 to 8.

Liquid chromatographic HPLC column filler Silica gel packing