News
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Dragging factor vs. symmetric factor vs. asymmetric factor What is the difference_
In chromatography experiments, we often encounter three terms: trailing factor, symmetry factor, and asymmetry factor, which are not well understood by many chromatographers and are sometimes confused. This article briefly describes the relationship and differences between the trailing factor, sy...Read more -
One guard column can solve most of the problems of the column and can greatly extend the use of the column time!
Why did the column break down after only three months of use? The sample is so dirty! The mobile phase is so harsh! The HPLC column is especially prone to breakage. What to do! I’ve already used in-line filters, and the pressure hasn’t increased, but the column breaks down fast. What ...Read more -
How to solve the “solvent effect”_
This paper aims to analyze the common phenomenon of the solvent effect in high-performance liquid chromatography, explore the principle of forming solvent effect, and propose corresponding solutions to provide corresponding guidelines for related practice. I. Overview The term “solvent eff...Read more -
How to search for pharmacopeia columns and their similar columns_
In the process of daily testing, the National Pharmacopoeia method is often used, in which the EP and USP methods give the packing of the stationary phase and the specifications of the hplc column, the Chinese Pharmacopoeia only the packing of the stationary phase, and now there are many columns ...Read more -
Analysis of condition filtering methods_
Concerning the preferred isocratic elution of high-performance liquid phase, the simpler the mobile phase ratio, the fewer buffer salts can be used. The simpler the elution method, the more common the hplc column, the better, but sometimes the separation of many impurities cannot be satisfied wit...Read more -
Why do your chemicals deteriorate so quickly_
Whether the chemical reagents will deteriorate in the storage process depends on internal and external factors, the internal factor is the physical and chemical properties determined by the chemical structure of the reagents themselves; the external factor is the environmental conditions in which...Read more -
Liquid chromatography at room temperature: When should a liquid chromatography method be set at the exact temperature_
In reversed-phase chromatography, increasing the column temperature usually decreases the retention of the analyte. In a method where column temperature is not controlled, what can go wrong if the temperature of the experimental environment changes? In early liquid chromatography, precise colum...Read more -
The most common 30 HPLC problems and solutions_
High-performance liquid chromatography is an important branch of chromatography. This method has become an important separation and analysis technique in chemistry, medicine, industry, agronomy, commodity inspection, and legal inspection. Taking the pharmaceutical field as an example: the Chinese...Read more -
Use, cleaning and storage of cyano column and amino column_
The amino column is a liquid chromatographic column used in normal and reversed-phase conditions. However, normal-phase solvents and reverse-phase solvents are immiscible and cannot be ignored. For newly purchased HPLC columns, first of all, please pay attention to opening the analysis and test i...Read more -
Twenty-one dos and don’ts for the mobile phase must-know_
The mobile phase is a key factor affecting liquid chromatography. An ideal mobile phase solvent for liquid chromatography should have low viscosity, good compatibility with the detector, easy access to pure products, and low toxicity. The main mobile phases used in HPLC are aqueous solvents, orga...Read more -
How many tips do you know about choosing a C18 column_
High-performance liquid chromatography (HPLC) is widely used in drug analysis, and the choice of the column directly impacts the drug’s results and quality control. The retention behavior of different C18 columns varies widely, and selecting a suitable column is crucial for analyzing sample...Read more -
How to effectively solve HPLC leakage problems_
For operators, liquid leakage is a distressing thing, because some leakage problems are relatively intuitive and easy to solve, but some leakage problems are more complex and need to be investigated in depth. Today, uHPLCs will talk to you about how to solve liquid chromatography leaks. In liqu...Read more -
An explanation of the main points of the drug impurity study methodology_
The standardization of impurity studies and their control within a safe and reasonable limit will directly affect the quality and safety of marketed drugs. Let’s learn more about impurity studies with uHPLCs. Source analysis of impurities By introducing the concept of Quality by Desi...Read more -
Do you know what high-speed counter-current chromatography is_
High-speed counter-current chromatography is a new analytical separation technique, mainly based on the differentiation of solutes between two immiscible solutions to distribute the differentiation factor to achieve the separation effect. The application of high-speed counter-current chromatograp...Read more -
HPLC Chromatography Accessories and Leak Handling_
Typical fittings consist of screws and ferrules designed to connect and seal the tubes. Despite their simple function, describing and using them is somewhat more complex. To describe a fitting exactly, several things need to be considered: the shape of the receiving hole (tapered or flat-low); wh...Read more -
Chromatographic quantitative internal or external standard method, how to choose_
One of the important roles of chromatography is to quantify the sample, and chromatography quantification is based on the weight of the component or its concentration in the carrier gas being proportional to the response signal of the detector. Common quantitative methods include area normalizati...Read more -
Laboratory water considerations_
With the development of science, laboratory instruments of all kinds are becoming more and more sophisticated, and the requirements for the reagents and water used in the testing process are becoming higher and higher. In particular, laboratory water, which is not used anywhere in the experimenta...Read more -
Major liquid mass spectrometers on|off procedure_
As the Chinese New Year holiday approaches, while preparing to enjoy the long holiday, uHPLCs would like to remind all laboratories to prepare for the shutdown of the liquid phase mass spectrometer coupler and to make the necessary backups of important experimental data before shutting down the m...Read more -
Must-know considerations for HPLC external standard methods_
The application of the HPLC method in research is content determination. What issues must be noted when applying the HPLC method to content determination? What precautions must be taken to calculate the content of external standards? In addition to the identification and related substances exa...Read more -
How does the HPLC spend its “life”_
High performance liquid chromatography in food safety detection work has an irreplaceable role. The author combined years of work experience and drew reference to the work of peer thinking, etc., from the user’s perspective of high performance liquid chromatography, the purchase of instrume...Read more
