The liquid chromatographic analysis most expected by chromatographic users: the highest resolution, the highest achievable sensitivity, and the fastest analysis speed. It can be said that being fast and good is the goal pursued by every industry. For this goal, the Ultra High Performance Liquid Chromatography (UPLC) was born. Compared with traditional HPLC, the speed, sensitivity and resolution of UPLC are 9 times, 3 times and 1.7 times that of HPLC respectively. It shortens the analysis time. At the same time, the amount of solvent is reduced and the analysis cost is reduced.
In addition to the above, what are the advantages or differences of uplc?
Specification
Compared with the 4.6 mm inner diameter X 250 mm length of an HPLC analytical column, UHPLC columns are generally shorter and narrower, approximately 2.1 mm inner diameter X 50 mm long. The length of the chromatographic column will affect the separation. Generally, the longer the length of the chromatographic column, the higher the pressure, the better the separation effect and the longer the analysis time. The length of the chromatographic column can be selected according to its own operational requirements. UHPLCS has various types of HPLC columns for your reference. It covers the widest range of applications and conditions, making your work easier and more efficient.
Operate pressure
Compared with the normal analytical range of high performance liquid chromatography (5000-6000 psi), the UPLC system can handle high pressure (15,000-18,000 psi). UPLC can use small particles with a particle size of less than 2μm to achieve the purpose of increasing performance. The filler of small particles requires the system to withstand higher pressure, so the uplc system is ultra-high pressure. Under such ultra-high pressure conditions, the requirements for chromatographic accessories are also higher. UPLC requires a chromatographic column with 1.7μm small particle packing, a low system volume infusion unit, and a system pressure that can withstand up to 15,000 Psi. For HPLC system, UHPLCS developed an on-line chromatographic filter suitable for ultra-high pressure liquid chromatography systems, with a pressure resistance of up to 15,000 psi, small dead volume, low back pressure, not easy to block, good corrosion resistance, and no leakage. The carefully designed product details and structure ensure that the product will not be easily separated or disintegrated during the experiment.
Analysis time
The use of UPLC can indeed significantly shorten the analysis time and improve the efficiency. Such as a certain related substance analysis method. Using HPLC to run once is 75 minutes, UPLC can be completed in 10 minutes, the analysis efficiency is increased by nearly 7.5 times.
Cost down
HPLC is an expensive separation technology. Not only the instrument itself is very expensive, but some daily consumables are also very expensive, such as chromatographic columns, high-purity solvents and so on.
Liquid chromatography has become an important separation and analysis technique in the fields of pharmacy, chemistry, medicine, industry, agronomy, commodity inspection and legal inspection. Among them, the emergence of ultra-high performance liquid chromatography has greatly promoted the development of the field of traditional Chinese medicine research. The complex components of traditional Chinese medicine and the difficulty of separation can be gradually solved by ultra-high performance liquid chromatography. The complex components of traditional Chinese medicine and the difficulty of separation can be gradually solved by ultra-high performance liquid chromatography. The 21st century is the century of science, and I believe that the application fields of liquid chromatography will be broader.
Post time: Jun-19-2021
