Liquid chromatographic columns are the core of liquid chromatographic analysis, and fillers have always been a hot project in the chromatographic field.The research of liquid chromatographic column can be described as the Central Plains competition, and the competition is very fierce. What changes have been experienced in the development of liquid chromatography columns?In terms of materials, glass was used at the beginning, but we all know that glass is very pressure-resistant, and the operating pressure of liquid chromatography is getting higher and higher. There is even ultra-high pressure liquid chromatography that uses glass cylinders. Is very inappropriate.NOW,The tubes of the liquid chromatography column are almost all made of metal
It is usually made of stainless steel, which has high strength and high hardness, is not easy to rust and corrodes, and has a wide range of applicable temperatures.UHPLCS ‘ liquid chromatography column tube is made of stainless steel. And nowadays liquid analytical columns are getting shorter and shorter, and many chromatographers use 250mm length columns. How long the column needs to be, or choose according to your own separation needs. UHPLCS provides 0mm, 50mm, 75mm, 100mm, 150mm, 250mm columns for options, and also provides 2.1, 3.0, 4.0, 4.6, 10, 20 , A variety of empty column tubes with 30 diameters, covering a variety of analysis and preparation needs.
When it comes to analytical columns, we have to mention chromatographic packing materials. Amorphous silica gel was used in the early packing, and a thin shell packing with a layer of silica gel coated on the surface of the glass beads was invented in the later period. This design helped to reduce the A term of the Van der Mute equation and increased the column efficiency by an order of magnitude. However, it also has the disadvantage that the stationary phase has a small capacity and is usually used to separate low-concentration samples.
Improving column efficiency has always been a hot topic in the chromatographic field, and fully porous spherical silica gel has been developed in the later period.Nowadays, more than 90% of current liquid chromatography packings are based on silica gel, and most of them are fully porous spherical.It also has many benefits, such as good mechanical strength, easy-to-control pore structure and specific surface area, better chemical and thermal stability, specific surface chemical reaction, and the surface is rich in silanol groups, which is easy for chemical bonding. Or modified, it is an ideal liquid chromatography packing matrix.UHPLCS packing are all porous spherical silica gels, and the purity of spherical silica silica is higher than 99.99%; optimization efficiency is improved by screening particle size; particle size and shape are tightly distributed to reduce back pressure; batch stability is consistent and repeatable Utilization; super compressive resistance; continuous and renewable retention time can ensure the process amplification from the laboratory to the application process; large quantities suitable for various purposes can ensure the number of products that can meet the processing needs.
Although silica gel has many benefits, it also has limitations. For example, it is resistant to acids and alkalis. Scientists are thinking of various ways to make up for such shortcomings, such as finding more alternative materials or improving the synthesis technology of silica gel. I believe that in the future, more new ideas and new methods will bring new developments.
Post time: Sep-13-2021
