The chromatographic guard column is very important in liquid phase analysis. The guard column can filter the internal pollution and sample contamination of the chromatographic system, so as to achieve the effect of protecting the chromatographic column. Especially for the analysis and detection of samples with complex matrices such as traditional Chinese medicine, natural products, synthetic compounds, etc., it is recommended to use the uHPLCs chromatographic guard column.
Because of the complexity of the matrix and the difference in sample solubility, the probability of sample precipitation and contamination of the chromatographic column is greatly increased, and the impact is greater during gradient elution. uHPLCs guard column can effectively filter contaminants and ensure the accuracy of experimental results.
It is also recommended to use a guard column in the analysis of biological samples. It can withstand the contamination caused by the irreversible adsorption of the sample before the chromatographic analysis column, thereby prolonging the life of the analysis column .So, how to select the LC guard column for liquid chromatograph? We will tell you today!
According to the nature of the analyzed sample, if you analyze some protein components and the sample is sensitive to metal ions, it is safer to use our PEEK guard column.
If you use Tetrahydrofuran in your mobile phase, you must use stainless steel, and even replace all the lines in your chromatography system with stainless steel, because Tetrahydrofuran is very corrosive to PEEK.
According to the different structure can be divided into rotary, direct and other styles of protection column. The advantage of the uHPLCs rotating guard column is that the column sleeve can be used repeatedly, and only the column core needs to be replaced regularly. It makes the pipeline not knotted, can increase friction, facilitate disassembly, extend the life of the analytical column, improve performance, and save costs.
uHPLCs direct-connected guard column can be directly connected to the entrance of the chromatographic column, and the hand-tight direct connection does not require other connecting pipes, which is easier to operate. The small dead volumn is positively and will not affect the analysis results.
Purchasing a high-quality, high-quality protection column that has a long service life and can protect expensive analytical columns will kill two birds with one stone. UHPLCS Scientific Instruments Co., Ltd. is a high-tech manufacturer focus on R&D and manufacturing of high quality chromatography consumables. As a local manufacturer of chromatography accessories with strong technical capabilities in China, we will do their best to provide more high-quality and more convenient products and services for the majority of scientific research workers, creating a better future hand in hand.
Post time: Dec-08-2021
