Liquid chromatography is widely used in sample analysis and determination in various fields, among which C18 column can analyze most compounds and is a common and entry-level column.When the column is in use, how should the daily maintenance and activation be done? uHPLCs takes everyone to understand!
uHPLCs recommends that before using a new liquid chromatography column, it is best to conduct a column performance test, record the test conditions and results, and use it as a reference for evaluating column performance changes in the future.Column efficiency is a very important factor to consider for chromatographic column. If you want good analysis results, you must maintain the chromatographic column and maintain its high column efficiency.Note when testing: your test environment and conditions should be in accordance with the conditions in the column delivery report, so that the results measured are comparable.
The column should be balanced before the sample is analyzed, and the injection analysis should wait until the baseline is stable. It usually takes 30 minutes to balance the mobile phase.Before using a new liquid chromatographic column, select appropriate reagents according to the characteristics of the column to clean out the reagents stored in the column. Special attention should be paid to the compatibility of the solvent used for cleaning and the solvent stored.
After daily use of the chromatographic column, be sure to pay attention to cleaning!If the mobile phase containing salt is used in the analysis, the mobile phase containing 10% methanol should be washed for 30 minutes after the completion of the analysis, and the residual salt in the column should be washed with methanol for another 30 minutes.Special attention: Do not rinse the column with pure water to prevent the packing from collapsing.
Newly purchased liquid chromatography columns require activation, and different columns require activation in different ways.For example, the most commonly used C18 column activation can be rinsed with methanol at a flow rate of 0.5 mL /min for more than 2 hours.There is a preservation solution in the column, and other impurities that may remain in the column are rinsed with methanol, so as not to affect the subsequent analysis work.The benefits of activation are many, can eliminate organic volatilization caused by bubbling.C18 column is bonded to C18, and activation of C18 column with methanol can make the bond arrangement better, which is more conducive to analysis and determination.It is better not to connect the detector when the column is activated, so as to avoid unnecessary chemical contamination.
It is better not to connect the detector when the column is activated, so as to avoid unnecessary chemical contamination.To extend the service life of the column, a protective column can be selected to filter out contaminants both inside the system and in the sample.Most reverse-phase chromatography columns have their own pH range. For example, the pH range of the constant-spectrum reverse-phase chromatography column is between 2-8. Try not to exceed this range to avoid damage to the column.
Post time: Mar-21-2022
