At present, 80% of the known compounds need to be analyzed by high performance liquid chromatography. It has been affirmed and used in the determination of components in various fields with the characteristics of fast, high efficiency, good sensitivity, high separation efficiency and wide application range. The Pharmacopoeia takes liquid chromatography as the standard analysis method for the determination of many components.
High performance liquid chromatography (HPLC) was established on the basis of classical liquid chromatography. The difference of HPLC is that it needs more chromatographic packing with smaller particle size, higher pressure pump and chromatographic column with better column efficiency.
The smaller size of the fillers means higher column efficiency, but it also brings the problem of increased pressure in the liquid system.The internal pressure of the system is high, which is a test for the pump, chromatographic column, protective column and pipeline.uHPLCs for such a high pressure liquid system, Constant quality has developed a number of high pressure resistant chromatography consumables, especially this 2.1# ultra high pressure UPLC protection column pre-column (Order NO. HPDGK-021040-1) can withstand the pressure of 15000psi, in such a high pressure environment, excellent product performance, no leakage, components do not dissolve, small dead volume.
The column is made of stainless steel with high strength and corrosion resistance, which is suitable for use in some mobile phases with strong corrosion, such as tetrahydrofuran.
The mobile phase of liquid chromatography is liquid, so it needs more consideration when choosing mobile phase as sample solvent. Mobile phase needs to choose chromatographic grade pure solvent, mobile phase and sample must be mutually soluble, if the solubility is different, precipitate compounds will cause serious consequences. The sample must be pretreated, although this step takes more time to be processed carefully, but if this step is not done well, it will play an important role in the quality of the experimental results.
High performance liquid chromatography (HPLC) can adjust the composition of mobile phase to make the retention value and selectivity of samples in the chromatographic column different, so as to achieve good sample separation effect. If you are analyzing high-boiling, polar, ionic, and macromolecular compounds, HPLC is a good choice for analysis.
In recent decades, HPLC method has been greatly developed, and analytical methods are increasingly mature. It has excellent performance in chemical engineering, environmental protection, environment, pharmacy, pharmaceutical, Traditional Chinese medicine, food, customs testing and other fields.
Post time: Mar-23-2022
