News
-
How does liquid chromatography column packing affect column performance_
The common packing materials in liquid chromatography columns are C1, C3, C4, C8, C18, C30, amino, cyano, silica gel columns, etc. Classification of packing materials in common chromatographic columns. a. Reversed-phase (with ion-pair) method C18 (octadecyl or ODS): good generality; strong reten...Read more -
Which of those quirky liquid chromatography columns have you used_
The production technology of gas chromatography columns has long entered a stable period, while liquid chromatography columns are still in the competition stage, with fierce competition and numerous technical innovations. Each company has its unique technique, and some new terms will pop up fro...Read more -
The little secrets of silica gel in liquid chromatography columns_
We all know that the packing material of the liquid chromatography column is generally chosen silica gel. Still, there are many requirements for silica gel packing material, summarized as follows. 1, the purity of silica gel 2, types of silica gel 3, the shape of the packing 4, the size of the pa...Read more -
Can I still use a long-lived column? COA_
Column analysis performance with COA. Students and researchers often ask us >Can I still use this lc column I bought 3-6 years ago? >I have heard from my senior that he has only used it 5 times, but his analysis is that it works well, right? >I found it in the warehouse, but I don’...Read more -
How many classic problems have you encountered with hplc columns_
What are the reverse phase column and normal phase column? “Inverse” and “positive phase liquid chromatography (HPLC) are the concept of” early put forward the concept of the bonded phase chromatographic column has not yet appeared. The stationary phase is coated on...Read more -
With all that nucleic acid testing, do you know what the red liquid in the nucleic acid tube is_
Have we measured nucleic acid so many times? Have we ever been “tube long”? For the nucleic acid sample tube in the liquid, do not know the screen in front of you will be curious? What exactly does it do? Why is it red? What is the red liquid in the nucleic acid tube? What does...Read more -
What is the meaning of “volume” in liquid chromatography_
Dead volume Dead volume definition The volume between the effective injection point and the effective detection point excludes the portion of the column containing the stationary phase. It consists of 4 parts: The volume of the inlet to the column line The gap between the stationary phase parti...Read more -
Comparison of the polarity of organic solvents_
Synthetic chemistry is inseparable from solvents, such as reaction media, HPLC column purification as eluent, extraction, etc. TLC spot plates require us to configure the unfolding agent, which then requires us to understand the nature and polarity of the various solvents. Polarity Polarity is ...Read more -
How to improve HPLC column efficiency_
1, Way to improve the HPLC Column efficiency of liquid chromatography Improving the efficiency of liquid chromatography can start from the following aspects. (1) reduce the flow rate of the mobile phase but will make the analysis time longer. (2) Reduce the stationary phase amount, but the col...Read more -
Why is the inner diameter of the column, not a whole number_
When people are doing the liquid phase, I wonder if someone is wondering the same question as I. Why are the internal diameters of the columns all some very fragmented numbers? For example, 4.6mm, and 2.1mm. I have never seen 5mm, 10mm, and other integers that look pleasing to the eye. I believe ...Read more -
Four of the causes of abnormal liquid chromatography baselines
I. Mobile phase impurities are leading to high and variable baselines. Impurities are highly soluble in the mobile phase in specific cases where they are not retained by the hplc column and contribute to higher-than-normal detector signals throughout the chromatogram. When using gradient elution,...Read more -
Differences in Types of Liquid Chromatography (HPLC)
High-performance liquid chromatography is a widely used analytical separation technique for quantifying, identifying, and separating the components of non-volatile liquid mixtures. The sample passing through the pores is called the “mobile phase.” When the mobile phase reaches the sta...Read more -
Liquid phase solvent effects also have a positive effect_ Learn about solvent focus
In the previous article, we talked about how when you do liquid phase analysis, if the intensity of the solvent you use to dissolve the sample and the mobile phase of the initial gradient conditions are relatively different, for example, if you use 100% acetonitrile to dissolve the sample. Still,...Read more -
Why do you use only methanol and acetonitrile as mobile phases in liquid chromatography_
I believe that students who use liquid chromatography deal with methanol and acetonitrile daily. But do you know why the mobile phases of liquid chromatography only use methanol and acetonitrile? Of course, the mobile phase of liquid chromatography also has water and some acids, bases, or buffer...Read more -
These are the things you need to know about UV-VIS detectors
In Autumn and winter, it is easy to encounter rain, snow, fog and other weather. We will find that in the fog or heavy rain environment, the same distance under the headlights emitted light than the normal weather transmission rate becomes lower as if the fog or heavy rain absorbs the light. &nbs...Read more -
Precautions in the use of liquid chromatography
I: Problems with the HPLC column Will using the same cleaning method for different types of columns affect the analysis of HPLC columns? Liquid chromatography columns will have different cleaning requirements depending on the brand, and you can look up the HPLC column manual or find informatio...Read more -
The larger the dead volume, the better how to get out of the peak_
A new batch of A brand liquid phase purchased inside a pharmaceutical plant to do a certain project out of the peak is bifurcation, which the engineer tossed many times at home, has not been solved. Next to another S brand of the old liquid phase to do the same project, the peak is no problem. &n...Read more -
Hard Knowledge: Three Simple Ways to Protect Your Liquid Chromatography Columns
Can our chromatography columns be indestructible and serve forever? We all know this is impossible, but there are ways to prevent columns from being too short-lived. Do we feel we are spending all of our lab funds on columns? Do we wonder why chromatography manufacturers can’t produce col...Read more -
Liquid chromatography column activation and maintenance precautions_
The performance of the column plays a decisive role in whether the measured substances can be well separated and determined. Therefore, special attention should be paid to the column’s activation, and the column’s routine maintenance is also important. Effective maintenance can prolon...Read more -
Bubbles in liquid chromatography analysis? Here’s how to fix it_
Ever had a meltdown over the constant bubbles when operating high-performance liquid chromatography? Have you ever fainted because the mobile phase still appears bubbles after an hour of ultrasonic shaking? Have you ever lost confidence in your work because no bubbles appear when the weather is f...Read more
