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Mass spectrometry and liquid chromatography
Mass spectrometry (MS) enables researchers to study many properties, such as the molecular weight, structure, properties, quantity and purity of the sample. When combined with liquid chromatography, it becomes a powerful analytical tool capable of extracting meaningful data on a range of compoun...Read more -
Select UHPLC or Not?
UHPLC is a specialized chromatographic method that operates faster, has better resolution, and uses fewer solvents than HPLC. UHPLC achieves this by using smaller columns filled with smaller particles, typically less than 2 µm in diameter. Researchers in biochemistry, cellular and molecular biolo...Read more -
HPLC Column Selection Guide
First, separation mode The primary task of selecting chromatographiy column is to select the separation mode. There are many methods to separate molecules, among which the most widely used methods include reversed phase, normal phase, hydrophilic interaction, ion exchange and size exclusion HPLC....Read more -
Guidelines for maintaining good condition of liquid chromatography columns
Keeping the HPLC column in tip-top condition will go a long way toward improving and standardizing your separation operations, while ensuring lc column life. You can take the following steps to achieve this goal. First, pay attention to cleaning. If you pay attention and clean regularly from th...Read more -
Choose HPLC or UHPLC Chromatography?
UHPLC is a specialised liquid chromatography method that runs faster, with better resolution and uses less solvent than HPLC. UHPLC achieves this by using smaller columns filled with smaller particles (typically less than 2 µm in diameter). Researchers in biochemistry, cell and molecular biology,...Read more -
Improved separation of large protein by ULTRA performance liquid chromatography-UHPLC
When UHPLC is first used for small molecule separation, good peak shape is obtained, but protein peak separation is not nearly as good, so it is usually not possible to reduce run time significantly. However, recent improvements to proteins allow UHPLC to provide better separation and shorter upt...Read more -
How to ensure effective HPLC column operation?
Samples introduced into AN HPLC column are often diverse and complex, and these samples may contain many ingredients that are incompatible with HPLC conditions. Injection of such samples without proper sample preparation or sample preconditioning may cause column clogging. For example, residual p...Read more -
High performance liquid chromatography troubleshooting
Leakage is a common problem in HPLC analysis. To minimize leaks in your system, try to choose hplc instrument parts from the same manufacturer. If an incompatible hplc joint is installed, problems may occur during separation and repeated connections may eventually result in a leak of the joint. I...Read more -
How to generate real liquid chromatography results?
As a liquid chromatography worker, your primary concern is to generate real experimental data that can be generally accepted and reproduced under the same conditions in any other laboratory. Even the most sophisticated tools will fail if globally accepted norms are ignored. System applicability i...Read more -
How to generate reliable HPLC results？
HPLC plays an important role in analytical research and quality control laboratory. Important decisions regarding the manufacturing process and publication of study results are made based on the high degree of reliability of the data generated by the HPLC system. Because HPLC is a multi-component...Read more -
Matters needing attention in high flux liquid chromatography sampling
In recent years, in order to reduce the analysis time and improve the analysis flux, the ultra-high performance liquid chromatography technology has made remarkable development. Ultra high performance liquid chromatography instrument can withstand higher system pressure and flow rate, and the ana...Read more -
How to maintain ultra performance liquid Chromatography (HPLC) columns?
Using high performance liquid chromatography (HPLC) to analyze samples is widely used in drug component analysis, food additive determination and environmental sample testing.Because liquid chromatography columns are the core equipment of many laboratories and are expensive, protecting columns fr...Read more -
Sample filtration is a key step in the preparation of chromatographic samples.
If the sample is not filtered before the experiment, the sieve plate of the column will be blocked and other problems, which will lead to the increase of back pressure and the degradation of the column performance.Ultimately, this will increase the time spent on troubleshooting and maintenance of...Read more -
How to choose the solvent filter of drug dissolution meter?
The filter of drug dissolution meter is an important part in drug quality control (QC) and drug development. When a sample is removed from the dissolution container, the dissolution test determines the rate at which the drug is released from the dosage form by measuring the amount of active pharm...Read more -
How to analyze high performance liquid chromatography?
Chromatographic analysis is very important in high performance liquid chromatography. You can think of the chromatogram as a two-dimensional graph with time and data on the horizontal and vertical axes. The stationary phase is a sponge-like column with many holes in it to allow the sample to flow...Read more -
Overview of basic knowledge of high performance liquid chromatography
At present, 80% of the known compounds need to be analyzed by high performance liquid chromatography. It has been affirmed and used in the determination of components in various fields with the characteristics of fast, high efficiency, good sensitivity, high separation efficiency and wide applica...Read more -
LC column maintenance and LC column conditioning methods_
Liquid chromatography is widely used in sample analysis and determination in various fields, among which C18 column can analyze most compounds and is a common and entry-level column.When the column is in use, how should the daily maintenance and activation be done? uHPLCs takes everyone to unders...Read more -
How to select the LC guard column for liquid chromatograph?
The chromatographic guard column is very important in liquid phase analysis. The guard column can filter the internal pollution and sample contamination of the chromatographic system, so as to achieve the effect of protecting the chromatographic column. Especially for the analysis and detection o...Read more -
Why use a liquid chromatography guard column?
Students who often use chromatographic columns for chromatographic analysis are confused. Why does the efficiency of the LC guard column decrease after a period of use? The decrease in column efficiency is mainly due to the contamination of the chromatographic column in the system from the sample...Read more -
AGEs components were determined by liquid chromatography
Liquid chromatographic analysis is a very good chromatographic analysis method in food analysis and determination. High performance liquid chromatography can be combined with many structural instruments to improve the sensitivity of detection. Advanced Glycation End Products AGEs are products tha...Read more
