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What are the Differences between Gas Chromatography (GC) and High Performance Liquid Chromatography (HPLC)?
Gas chromatography (GC) is an ideal technique for separating and identifying low molecular weight gases or volatile liquids. It has been widely used in industry, agriculture, national defense, construction and scientific research. Gas chromatography can be divided into gas solid chromatography an...Read more -
Why do HPLC Mobile Phase Solvents Need to be Filtered and Deaerated?
To answer this question, let’s first understand what a mobile phase is. Simply put, a mobile phase is a fluid in which a carrier carries a substance into and through the stationary phase. The mobile phase can be either a water-organic mixture of the buffer combined with an organic solve...Read more -
Misconceptions about HPLC and UHPLC Columns
Misconception 1：HPLC column can not be backflushed. This is incorrect. In general, Liquid chromatographic columns are designed to withstand pressures well above the maximum operating pressures. When replacing the column, the backflush can clean the column head of some substances with strong adso...Read more -
How to Recover the Performances of HPLC Column?
It is well-known that HPLC column has a limited lifetime. As time goes by in use, its performance begins to decline. You can tell it from the peak shape and separation between peaks, the irregular increasing of column back-pressure and the presence of ghosting peaks. Chromatographic columns are e...Read more -
What are the Potential Applications of HPLC in Clinical Laboratories?
HPLC plays an important role in the isolation and quantification of biomarkers in different body fluids in clinical laboratories. Compared to other conventional detectors(such as UV, fluorescent and electrochemical detectors), HPLC tends to do quality sensitive testings. With the development of p...Read more -
What are protective columns and what role do they play in HPLC analysis?
Effectivity of the HPLC system depends on the mobile phase and the sample being free from chemical impurities or solid suspensions. The discussion on the handling and use of HPLC mobile phase mainly focuses on the measures to be taken in the preparation and use of mobile phase. Discussions on the...Read more -
How are analytical scale and preparative scale HPLC different?
HPLC is a versatile analysis tool that affords separation and quantification of large range of chemical compounds.It finds unlimited applications in basic research, pharmaceuticals, foods, polymers and other similar applications. The first and foremost thing to keep in mind is that the basic prin...Read more -
Want the steady operating of HPLC system? You must learn to to get “flu shot”!
For liquid chromatography that need a full day operation and analysis, it is easy to cause failure for a long time operation. So we can take some preventative maintenance measures in advance to reduce the probability of instrument failure and make the normal operating of the system. Just as the r...Read more -
How to choose the column length and inner diameter of Liquid chromatography column?
Liquid chromatography column is made of column, Pressure cap, cutting sleeve(sealing ring), frit cap（filter disc), connector, thread and so on. There are many materials for column tubes, such as polymer, glass, stainless steel and so on. The highly recomment is stainless steel material. Because ...Read more -
Importance of HPLC grade solvents in HPLC analysis
In addition to the routine maintenance of liquid chromatograph, the high purity solvent also can be protecting the liquid chromatography system and increase the reliability of the results. HPLC grade means the purity of chemical reagents. It is the purity of reagents that can be used in high-perf...Read more -
What should be attention when using process of liquid chromatography?
Not only is LC expensive, but also the Chromatography consumables. It will cause much damage if some operations aren’t be attention. Now let’s learn and remember them. 1.The quality of LC It is better to internal calibration of the instrument at regular intervals. 2.Operator As an operat...Read more -
We were in P-MEC China 2020 exhibition
12.16-18，P-MEC China 2020 was held in Shanghai New International Expo Cente.UHPLCS team has come to Shanghai to attend this exhibition. ...Read more -
Analysis on the Import and Export Status of China’s Inspection and Testing Instruments and Equipment in 2019
China’s inspection and testing equipment market demand is vast, and the price growth rate drops. According to statistics from the State Administration of Market Supervision and Administration, from 2014 to 2018, the number of instruments and equipment in the inspection and testing service i...Read more -
In July, 3 new food supplementary inspection methods were announced, all of which are liquid chromatography！
On July 29, 2020, the General Administration of Market Supervision issued the “Determination of Trisodium Trimerthiocyanate in Wheat Flour”, “Determination of Benzohydroxamic Acid in Wheat Flour and Its Flour Treatment Agent”, and “Determination of Kojic Acid in Whea...Read more -
What is the chromatographic colum?
Chromatography is a laboratory technique for the separation of a mixture. A Chromatography column is a device used in chromatography for the separation of chemical compounds. Five methods will use columns: GC, LC, IEC, SEC and chiral chromatography. Today, we share two methods between them-Gas th...Read more -
When Liquid Chromatography meets the ghos
Ghost peak is one of the common questions in Liquid chromatography, many elements will lead to the ghost peek. Any improper operation in the process of the liquid chromatography will cause the ghost and impact the accuracy of any result. According to the frequency of ghost peaks, the main factors...Read more -
What are the application areas of liquid chromatography?
Chromatography is a powerful separation technology that is combined with MS and other detection means has a great development. The chromatography application is divided into Preparative Chromatography and Analytical chromatography according to the purpose. The purpose of preparative chromatograph...Read more -
The analytica China had been successfully concluded
Analytica China is the International trade fair for laboratory technology, analysis and biotechnology in China. From 16. Nov to 12. Nov 2020, analytica China was held in Shanghai New International Expo Center E2-E7 Hall. As a high-tech manufacturer focused on R&D and manufacturing of high qua...Read more -
What is choromatography?
For many people, choromatagraphy is strange and mysterious. But for some scientific researchers who engaging in scientific experiments and testing, it likes a brother that always in lab. ‘Chromatography’ is an analytical technique.It is a process for separating components of a mixture. There are...Read more -
UHPLCS丨All the chromatography knowleges that you want to know are here
Since the invention of chromatography in the early 20 century, it has become the most important separation science and widely used in lots of areas such as petrochemical, organic synthesis, physiology, biochemistry, medicine & health, environment protection even space exploration and so on. C...Read more
