In the last new, we discussed “What Are The Differences Between Gas Chromatography (GC) And High Performance Liquid Chromatography (HPLC)?”(Click here to see it ). Today, we are talk about the common fuction between HPLC and GC, in-depth study of the commonality between them.
1.Common basis
Separation of components of a mixture, their identification and quantification is the common underlying objective of both the techniques. This is achieved through a choice of columns, mobile phases, stationary phases, detectors and operating conditions.
2.Role of Mobile Phase
When using the HPLC and GC instruments all need a mobile phase to transport the components to be detected to the detector sequentially. Although they working on the same principle, the mobile phase of HPLC is liquid, the GS is gas. Want to know more about the mobile phase, please click it to look -“Why Do HPLC Mobile Phase Solvents Need To Be Filtered And Deaerated? Misconceptions About HPLC And UHPLC Columns”
3.Sample analysis
Both GC and HPLC are capable of analysis of liquids and dissolved solid mixtures though gaseous mixtures are also routinely analyzed using GC. Sample introduction techniques are common generally common.A gas tight syringe is sufficient for introduction of liquid samples. For gases Teddler bags or auto sampler (manual or automated operation) are used.In HPLC systems the mobile phase is moving under higher pressure so direct syringe injection is not possible. A syringe is used to fill a fixed volume loop in the manual sample introduction valve and with the change of the valve position the entire sample inside the loop is carried by the mobile phase into the column. Auto samplers are a great help for unattended operation when the number of samples is large.
4.Chromatogram
Both GC and HPLC chromatograms look similar. The retention time on the horizontal axis is a characteristic, and a retention time corresponds to a substance. The electrical signal on the vertical axis corresponds to the concentration.
5.Common Application Softwares
Mostly manufacturers supply common HPLC and GC application software as peak analysis and data treatment are common .
6.Instrument composition
The same instrument accessories such as injectors, chromatographic columns, and detectors will be used. Autosampler can be adopt to save time and labor cost when the separated ingredient is more and Cumbersome. Although the HPLC and GS need to use the Chromatography column, the type of chromatographic column and the packing material are different. The gas phase includes packed columns and capillary columns; the liquid phase includes normal phase chromatographic columns and reversed phase chromatographic columns.
Both GC and HPLC are the pillar stones of chromatographic analytical laboratories and while it is important to know the differences between them it is equally important to understand the similarities so as to decide on the right method for a particular application in hand. UHPLCS scientific Instruments Co.,Ltd.is a high-tech manifacturer focused on R&D and manufacturer of high quanlity chromayography comsumables.Our strategic partners have customers in chemical, pharmaceutical, biological, medical, food, materials, environmental protection, agriculture, inspection, scientific research and other industries.
Post time: Apr-09-2021
