If the role of the chromatographic column in liquid chromatography analysis is like the human “brain”, then the chromatography packing is like our human “heart”. The quality and accuracy of liquid phase analysis have a very important relationship with fillers. There are many types of liquid chromatography fillers, mainly divided into polymer fillers, silica gel matrix fillers and other inorganic fillers. Among them, silica-based fillers are widely used in various liquid chromatography analyses.
What are the benefits of silicone fillers? The silica gel matrix has high purity, and the pH value is usually between 2-8. The pH value of some special silica gel bonded phases can be stabilized at pH 1.5-10. At the same time, it also has a relatively high specific surface area, which has strong retention capacity, column capacity and resolution; high mechanical strength, which can ensure that the column efficiency can be stable under ultra-high pressure operating pressure; high-strength particles make The back pressure of the analytical column is lower and the service life is increased.
UHPLCS silica gel chromatographic packing strictly monitors the quality, has a nearly perfect spherical surface and almost the same particle size, and strictly controls the problem of column pressure rise caused by the existence of tiny particles. High purity, metal impurities <10ppm, with high column efficiency, high reproducibility, and low column pressure.
Regardless of whether it is used in preparative or analytical grade chromatography, or in reversed-phase normal phase chromatography, uHPLCs has corresponding chromatographic fillers. The particle size range: 1.8, 2, 3, 5, 10 and larger particles can be used. select. The uHPLCs USHA Sil chromatographic column adopts high-purity spherical silica packing, which can be used to separate polar and basic organic compounds, such as vitamins and steroids. The uHPLCs USHA NH2 chromatographic column has strong polarity and can be used for both positive and negative. The amino functional group provides retention and allows the analysis of polar compounds under normal phase elution conditions. Acetonitrile and water can be used to analyze organic compounds in monosaccharides and polysaccharides.
All in all, there is no absolute good or bad word for chromatographic packing. It mainly depends on the analyte. Choose the suitable chromatographic column and packing type according to the characteristics of the analyte.
Post time: Oct-19-2021
