Chromatography is a powerful separation technology that is combined with MS and other detection means has a great development. The chromatography application is divided into Preparative Chromatography and Analytical chromatography according to the purpose.
The purpose of preparative chromatography is to separate the mixture and get a certain quantity of pure components. It includes purification of organic synthesis products, separation and purification of natural products and preparation of deionized water. Compared with the purification separation technology such as Recrystallization, chromatography can complete the separation of mixtures in one step. But the yield of chromatography purification is limited, just suitable for laboratory.
Analytical chromatography is the typical chromatography method used to identify the components of a mixture and their proportions. The main purpose of analytical chromatography is the qualitative and quantitative analysis of the components of a mixture. The qualitative analysis chromatography includes thin layer chromatography, paper chromatography and so on. The quantitative analysis chromatography includes gas chromatography, HPLC and so on. Chromatography used in the analysis made the process of separation and determination in one step. It reduces the difficulty of mixture analysis and shorter the analysis cycle, which is a mainstream analysis method.
The application area of liquid chromatography is as below:
Application in life sciences
Amino, acid, polypeptide, protein, Nucleobase, Nucleosides, Nucleotide, RNA, DNA and other important living matters and be purified, analyzed and measured by liquid chromatography.
The purification and quality control of synthetic drugs, separate preparation and purity determination of Chinese herbal medicine active ingredient, and separation analysis in pharmacokinetic research of clinical medicine all adopt liquid chromatography. Except for polymer, about 80% of drugs can be separated and purified by liquid chromatography.
Food analysis
The composition of the food itself, especially the analysis of nutrients, such as sugar, organic acids, vitamins, proteins, amino acids and fats.
Food additives
Such as the analysis ofcorrosion remover, Antioxidants，synthetic dyestuff，sweetening agent.
Food contaminants
Such as the analysis of pesticide residue, aflatoxin and so on.
Environment monitoring
Used for the separation and analysis of organic pollutants with large molecular weight, low volatility and poor thermal stability. Such as Polycyclic aromatic hydrocarbons, Phenols, PCBs, Phthalates, Benzidines, Anionic and non-ionic surfactants, Organic pesticides and so on. It has become an important analysis method of the environmental science field.
Analysis of Fine Chemical Products
Used for some organic compounds with higher molecular weight and higher boiling point,such as the high – carbon aliphatic or aromatic alcohols, aldehyde, ketone,ether，acid,ester and other chemical material all can be analysied by liquid chromatography .
The liquid chromatography has widly using application. The price of a liquid chromatograph can range from tens of thousands of yuan to hundreds of thousands of yuan. There will be huge loss if the liqud chromatography can not be well maintenance. Chromatography supplies as the frequently use and replace elements of chromatographic instrument. It will lower the damage to choose a durable and guaranteed quality product. UPLCS liquid chromatography equipment made of stainless steel and peek, has advantage of heat-resistant,high pressure resistant,good toughness and high filtration accuracy.
UHPLCS solvent suction filter has advantage of non-bubble, 99% solvent efficiency. Compatiable with variety of mobile phare pipelines.
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Post time: Nov-27-2020
