HPLC plays an important role in the isolation and quantification of biomarkers in different body fluids in clinical laboratories. Compared to other conventional detectors(such as UV, fluorescent and electrochemical detectors), HPLC tends to do quality sensitive testings. With the development of packing materials, sub-micron surface porous silica particles has been introduced in UHPLC. Without facing high back pressure, the separation efficiency is improved.
The system pressure of high performance liquid chromatography is a little high. Chromatographic column is the main component that produces system pressure. High back pressure affects many things. For example, too high back pressure that exceeds the tolerance of the flow pool can cause damage to the circulation pool. The uHPLCs’s uniform particle size of a liquid chromatography column ensures the stable flow of the mobile phase. What’s more, it can ensure a low column pressure to chromatographic column.
UHPLCs analytical guard column also has the advantages of low back pressure, no leakage, less dead volume, etc. It can reduce some of the pressure in the liquid chromatography system.
In addition, with the introduction and combination of other advanced techniques, the working methods of liquid chromatography such as efficient automatic sample injectors, reduced detector cell size, and improvements in data collection and analysis have been optimized to some extent. Many laboratories need to generate multiple parameter reports on hundreds of samples per day. These methods can save working time and improve work efficiency.
Clinical laboratories typically handle hundreds of samples of biological fluids, such as blood, urine, and other body fluids. In order to quickly handle such a heavy workload without compromising the quality of the analysis, an efficient extraction process combined with rapid analysis is needed. The way a patient is treated has a lot to do with the accuracy of the reports, so quick and accurate reporting is a priority.
This article briefly discusses some common applications of HPLC analysis in clinical laboratory. Biological samples need to be preprocessed and processed to eliminate interference from other components, especially in the case of fluids such as blood. Solvent extraction, ultrafiltration and solid phase extraction are used to prevent internal blockage in HPLC column. To avoid HPLC column blockage, some liquid analysis consumable accessories can be used like adding a pre-column
In addition to installing guard columns, in-line filters can be installed to prevent column blockage. uHPLCs in-line filters is made of high quality clean stainless steel parts. These filters are designed to reduce contamination and blockage for column frit caps.
Post time: Mar-06-2021
