Not only is LC expensive, but also the Chromatography consumables. It will cause much damage if some operations aren’t be attention. Now let’s learn and remember them.
1.The quality of LC
It is better to internal calibration of the instrument at regular intervals.
2.Operator
As an operator of LC, it is necessary to read the manuel of LC. Master the basic use and operation precautions, understand and regularly carry out the maintenance of the instrument, and understand some basic fault problem discovery and handling skills.The chromatograph is an expensive instrument. In many cases, it is caused by improper use of the operator to cause irreversible blockage and damage of the chromatographic column, excessive system pressure, abnormal ghost peaks, etc. When using the instrument, you must be careful and responsible. The operation steps should be followed step by step. Don’t be too cumbersome to do less or omit. Clean the solvent filter, solvent bottle, etc. after using the instrument to keep it in normal use next time.
3.During operation
Not filter mobile phase
We need to remove impurities, particles and bacteria in the mobile phase in advance, because these impurities, particles and bacteria will wear the plunger, sealing ring, cylinder and check valve.In addition, unfiltered solvents and solvent bottles are not replaced in time. Microorganisms generated by cleaning will reduce the life of the solvent inlet filter and affect the performance of the pump.It usually takes about 3 months to replace the solvent filter head, or when you find that there is no solvent or only a small amount of solvent dripping from the tube, your solvent filter head will need to be replaced because it is blocked.
The solvent filter is mainly used to filter impurities, particles, bacteria, etc., so long-term use will cause clogging, and if the solvent filter’s sampling efficiency is not high, it will also cause waste of solvent.UHPLCS 2μ, 5μ, 10μ, 20μm solvent inlet suction filter protects the pump, one-way check valve, sampler, and chromatographic column from harmful particle contamination. The suction filter head is small in size and can be used in a variety of storage tank containers with small bottle mouths. It will not introduce air into the system, and no bubbles will enter the mobile phase pipeline. The solvent extraction utilization rate of the liquid storage bottle can reach more than 99%, which greatly reduces the waste of the solvent at the bottom of the bottle due to the inability to clean vacuum suction.
When the pump is working
When the pump is working, take care to prevent the mobile phase in the solvent bottle from being used up, otherwise, the empty pump will wear the plunger, cylinder, or sealing ring, and eventually cause leakage. The working pressure of the pump must never exceed the specified maximum pressure, otherwise, it will deform the high-pressure seal ring and cause liquid leakage.
The pump is not cleaned in time after using
The mobile phase must not contain any corrosive substances or impurities, and the mobile phase containing buffers should not remain in the pump, especially in the pump for a long time. You must add pure water to the pump to fully clean the pump and save it for the next use.
Mobile phase
The flow should be degassed first, so as not to generate bubbles in the pump and affect the stability of the flow. If there are a lot of bubbles, the pump will not work properly. What if there is no flow of the mobile phase and no pressure indication? There may be a large amount of gas in the pump. At this time, you can open the pressure relief valve to make the pump run at a larger flow rate (such as 5ml/min) to exhaust the bubbles. You can also use a 50ml syringe to help pump out the gas at the pump outlet. Another possible cause is that the seal ring is worn and needs to be replaced.
The above are some of the key factors that need attention in the operation of liquid chromatographs.Of course, the liquid chromatograph is a complex analytical instrument, composed of many accessories, not only the above factors need attention.However, checking the instructions and continuously learning to master more points of use is the way to use and operate the liquid chromatograph correctly.And it is not only a problem of operating methods, but in many cases we can use some chromatographic consumables and accessories to protect our instruments and solve many failures.For example, how to avoid ghost peaks? We can use the UHPLCS ghost peak trapping column, install the ghost peak trapping column between the gradient mixer and the autosampler, which can not only remove impurities in the mobile phase, but also effectively trap the pipeline and mixer Impurities in.
UHPLS Scientific Instrument CO., Ltd, is a high-tech manufacturer focused on R&D and manufacturing of high quality chromatography consumables. We have rich production experience and strong manufacturing technology capabilities in non-bubble solvent inlet filter with high purification capacity,0.1um inner surface roughness HPLC guard column tube, ghost peak removal column ,tubingh connector thread filtting,etc.
Post time: Jan-09-2021
