HPLC Guard Columns Manufacturer
Retains chemicals and particulates that cause clogging of the column frit, collapse of the column head and reduced column efficiency.
Catalogue
Note: The following factors are considered in the selection of guard columns;
1. unless special circumstances, generally choose the packing and analytical HPLC column matrix in the same protective column.
2. Select the size of the guard column can be calculated by the HPLC column volume; the volume of the guard column can be 5% to 10% of the HPLC column volume; such as 4.6 * 250mm LC column volume of about 4mL, and we suggest that the volume of the guard column should be select between 0.2~0.4mL. At the same time, the inner diameter of the guard column should be the same or equivalent to the inner diameter of the HPLC column.
HPLC Guard Columns retains those strongly retained, non-adsorbable compounds. The filtration of the sample and mobile phase maintains the adsorption capacity of the column for chemical contaminants, enabling the column to be maintained for a longer period of time.
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HPLC Column Packing |
Physical and Chemical Parameters |
Features and Applications |
Specifications of HPLC Column (mm) |
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Particle Size (μm)
|
Pore Size ( A) |
Carbon Content (% ) |
PH Stability |
End-apping |
Separation mode |
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USHA C18 |
1.8 2 3 5 10 20 30 or even bigger particles |
70 100 120 200 300 etc. |
17 |
2~8 |
Yes |
Reversed-phase |
- High selection and high separation.
- Widely used in in the analysis of polar and hydrophobic substances, the first choice of all kinds of compounds. |
2.1x30 2.1x50 2.1x75 2.1x100 2.1x150 3.0x50 3.0x100 3.0x150 4.6x30 4.6x50 4.6x100 4.6x150 4.6x250 10x50 10x100 10x150 10x250 20x50 20x150 20x200 20x250 20x300 30x150 30x250 50x150 50x250 |
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USHA C18-G |
18 |
1~12 |
One-End-capping |
Reversed-phase |
- High selection and high separationl High- efficiency column
- A wide range of pH values, the first choice of all insulin substances - Widely used in the analysis of polar and hydrophobic substances |
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USHA C18-BIO |
17.5 |
2~10 |
Double End-capping |
Reversed-phase |
- Specially designed for the separation of various alkaline compounds.
- The pH tolerance range is 2~9. |
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USHA C18-T |
19 |
1.5~11 |
Double End-capping |
Reversed-phase |
- Designed for selective separation, aim for high quality separation and purification. | |||
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USHA C18-N |
16 |
2~8 |
No |
Reversed-phase |
- Unique bonding mode, can achieve 100% water phase condition. | |||
|
USHA C18-AQ |
13 |
2~8 |
Yes |
Reversed-phase |
- C18 and polar group. | |||
|
USHA C18-A |
10 |
2~8 |
Yes |
Reversed-phase |
- Low carbon content, suitable for separation of strongly polar and hydrophilic compounds. | |||
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USHA C8 |
10 |
2~8 |
Yes |
Reversed-phase |
- C8 has a relatively low hydrophobicity compared with C18 stationary, suitable for the separation of most hydrophobic compounds, more suitable for separating compounds with strong adsorption on C18 column. | |||
|
USHA C4 |
3 |
2~8 |
Yes |
Reversed-phase |
- Suitable for the separation of polar and hydrophobic substances in C18 and C8 separation for too long. | |||
|
USHA Phenyl (USHA phenyl column) |
12 |
2~8 |
Yes |
Reversed-phase |
- π- π interaction shows selectivity difference from that ofC18. | |||
|
USHA NH2 (USHA amino column) |
4 |
2~8 |
Yes |
Normal-phase Reversed-phase |
- Strong polarity can be both positive and negative; The amino functional group provides a retention that allows the analysis of polar compounds under normal phase elution conditions. - Organic compounds in monosaccharides and polyglycans/ olefines/aromatics can be analyzed by acetonitrile and water. - In the buffer of low pH value, there is weak anion exchange which can separate some negatively charged molecules. |
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USHA CN (USHA cyan column) |
6 |
2~8 |
Yes |
Normal-phase Reversed-phase |
- Medium polarity cyanogroup, can be used in both positive andnegative phases.
- The hydrophobicity is relatively low in the stationary phase of reversed-phase chromatography and shows a different selectivity from that of C18 due to the π-electron interaction of the cyanide group. - Suitable for separation of components that have been separated for too long on C18, and suitable for the occasion where is very difficult to optimize the chromatogram on C18. - When very hydrophobic compounds cannot be eluted using standard C18 and C8 reversed-phase column, the most polarized inverting column can be a cyanide column. |
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USHA Diol |
5 |
2~7 |
No |
Normal-phase Hilic |
- Separation of polar and basic compounds using silanol residues. | |||
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USHA SiL |
3 5 7 9 10 20 30 or even bigger particles |
70 120 150 200 300 etc. |
-- |
1~6 |
Yes |
Normal-phase Hilic |
- <10ppm high purity spherical silica gel, very low metal impurities. - Can be used to separate polar and basic organic compounds, such as vitamins, steroids, and many other drug molecules. |
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|
USHB C18 |
17 |
2~8 |
Yes |
Reversed-phase |
- Exellent stability and repeatability, full cover bonded silica gelwas used as packing.- High selectivity and high separation. | |||
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USHB C18-BIO |
20 |
1.5~10 |
Double End-capping |
Reversed-phase |
- Specially designed for the separation of all kind of alkali compounds, the pH tolerance range can be 1.5-10. | |||
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USHB C18-BP |
14 |
2~7.5 |
Yes |
Reversed-phase |
- Low carbon content, suitable for separation of hydrophilic and polar compounds, and 100% pure water mobile phase. | |||
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USHB C8 |
10 |
2~8 |
Yes |
Reversed-phase |
- Suitable for separating most hydrophobic compounds | |||
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USHB SiL |
-- |
1~6 |
No |
Normal-phase Hilic |
- High purity spherical silica gel (metal impurities < l0ppm), withhigh column efficiency and good peak shape characteristics.
- Can be used to separate polar and basic organic compounds. |
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Why is it necessary to have an analytical guard column?
Various reagents are used in the mobile phase of HPLC and some insoluble or impure substances can be mixed with them. Most of the insoluble material can be removed by filtering the mobile phase with a sieve plate or membrane filter, but very fine particles can pass through the filter. There is also the possibility of insoluble or impure substances falling in from the air or from containers, the human body etc. after the mobile phase has been modulated. Depending on the purpose of the analysis, these insoluble or impure substances may contaminate the column, clog it, or adversely affect the analysis. This can be prevented by installing an analytical protection column in front of the column, which protects the column.
Analysis of the role of guard columns
The analytical guard column serves to pre-trap substances that will be firmly adsorbed by the column and cannot be washed by the mobile phase. The packing of the analytical protection column is usually the same as that of the chromatographic column, as the damage to the analytical column is in most cases only a small part of the packing on the inlet side of the column. uHPLCs recommends that you make effective use of analytical guard columns to extend the life of the column.
What kind of liquid chromatography guard column do you need?
Need a high-quality guard column
Need a good-priced guard column
Need a standard guard column
Need a guard column with consistent packaging
Need a guard column with guaranteed after-sales service
Our products can fully meet your requirements!
You Are Welcome to Contact us by Email by sales@uhplcs.com with any questions for our HPLC Guard Column Cartridge, we will get back to you within 48 hours.
