Chromatographic Column Packing

Chromatographic Column Packing

Chromatographic Column Packing

HPLC ANALYTICAL GRADES

Super Pure Spherical silica product line is dedicated to analytical HPLC and UHPLC where high efficiency and high mechanical strength is required.

HPLC SEMI-PREPARATIVE & FLASH GRADES

Flash chromatographt is a quick and easy way to separate complex mixtures of compounds. The silica gel is specifically designed to increase cartridge efficiency while exhibiting moderate back-pressure; and therefore improving resolution.

PREPARATIVE & FLASH GRADES

The silica gel is fully dedicated to preparative HPLC. The SMB line offers significant advantages including easy packinghigh efficiency, and high capacity, thus offering economic advantages for large-scale processes.

INDUSTRIAL LC GRADES

Industrial Liquid Chromatography plays an essential role in many industrial sectors, this technique is uesd in the chemical, agrochemical, cosmetic, veterinary, pharmaceutical, etc., to purify natural extracts, organic intermediates, drug substances,etc...

色谱填料-英文

Features & Benefits

>SPS purity SiO2 is higher than 99.99%

>Selection of particle sizes to optimize efficiency

>Tight distribution of particle sizes and spherical particle shape to reducr back-pressure

>Lot-to-Lot consistency for reproducible performance

>Superior pressure strength

>Consistent and reproducible retention times to allow direct scale-up from laboratory through process applications

>Large bulk quantities for all applications available, secutrd lots offered for process use

HPLC Column

Packing

Physical and Chemical Parameters

Features and Applications

Specifications of HPLC Column (mm)

Particle Size

 (μm)

 

Pore Size

 ( A)

Carbon Content 

(% )

PH Stability

End-apping

Separation mode

USHA C18

1.8

2

3

5

10

20

30

or even

bigger

particles

70

100

120

200

300

etc.

17

2~8

Yes

Reversed-phase

- High selection and high separation.

- Widely used in in the analysis of polar and hydrophobic substances, the first choice of all kinds of compounds.

2.1x30

2.1x50

2.1x75

2.1x100

2.1x150

3.0x50

3.0x100

3.0x150

4.6x30

4.6x50

4.6x100

4.6x150

4.6x250

10x50

10x100

10x150

10x250

20x50

20x150

20x200

20x250

20x300

30x150

30x250

50x150

50x250

USHA C18-G

18

1~12

One-End-capping

Reversed-phase

- High selection and high separationl High- efficiency

- A wide range of pH values, the first choice of all insulin substances

- Widely used in the analysis of polar and hydrophobic substances

USHA C18-BIO

17.5

2~10

Double End-capping

Reversed-phase

- Specially designed for the separation of various alkaline compounds.- The pH tolerance range is 2~9.

USHA C18-T

19

1.5~11

Double End-capping

Reversed-phase

- Designed for selective separation, aim for high quality separation and purification.

USHA C18-N

16

2~8

No

Reversed-phase

- Unique bonding mode, can achieve 100% water phase condition.

USHA C18-AQ

13

2~8

Yes

Reversed-phase

- C18 and polar group.

USHA C18-A

10

2~8

Yes

Reversed-phase

- Low carbon content, suitable for separation of strongly polar and hydrophilic compounds.

USHA C8

10

2~8

Yes

Reversed-phase

- C8 has a relatively low hydrophobicity compared with C18 stationary, suitable for the separation of most hydrophobic compounds, more suitable for separating compounds with strong adsorption on C18 column.

USHA C4

3

2~8

Yes

Reversed-phase

- Suitable for the separation of polar and hydrophobic substances in C18 and C8 separation for too long.

USHA Phenyl

(USHA phenyl column)

12

2~8

Yes

Reversed-phase

- π- π interaction shows selectivity difference from that ofC18.

USHA NH2

(USHA amino column)

4

2~8

Yes

Normal-phase

Reversed-phase

- Strong polarity can be both positive and negative; The amino functional group provides a retention that allows the analysis of polar compounds under normal phase elution conditions.

- Organic compounds in monosaccharides and polyglycans/ olefines/aromatics can be analyzed by acetonitrile and water.

- In the buffer of low pH value, there is weak anion exchange which can separate some negatively charged molecules.

USHA CN

(USHA cyan column)

6

2~8

Yes

Normal-phase

Reversed-phase

- Medium polarity cyanogroup, can be used in both positive andnegative phases.

- The hydrophobicity is relatively low in the stationary phase of reversed-phase chromatography and shows a different selectivity from that of C18 due to the π-electron interaction of the cyanide group.

- Suitable for separation of components that have been separated for too long on C18, and suitable for the occasion where is very difficult to optimize the chromatogram on C18.

- When very hydrophobic compounds cannot be eluted using standard C18 and C8 reversed-phase column, the most polarized inverting column can be a cyanide column.

USHA Diol

5

2~7

No

Normal-phase Hilic

- Separation of polar and basic compounds using silanol residues.

USHA SiL

3

5

7

9

10

20

30

or even

bigger

particles

70

120

150

200

300

etc.

--

1~6

Yes

Normal-phase Hilic

- <10ppm high purity spherical silica gel, very low metal impurities.

- Can be used to separate polar and basic organic compounds, such as vitamins, steroids, and many other drug molecules. 

USHB C18

17

2~8

Yes

Reversed-phase

- Exellent stability and repeatability, full cover bonded silica gelwas used as packing.- High selectivity and high separation.

USHB C18-BIO

20

1.5~10

Double End-capping

Reversed-phase

- Specially designed for the separation of all kind of alkali compounds, the pH tolerance range can be 1.5-10.

USHB C18-BP

14

2~7.5

Yes

Reversed-phase

- Low carbon content, suitable for separation of hydrophilic and polar compounds, and 100% pure water mobile phase.

USHB C8

10

2~8

Yes

Reversed-phase

- Suitable for separating most hydrophobic compounds

USHB SiL

--

1~6

No

Normal-phase Hilic

- High purity spherical silica gel (metal impurities < l0ppm), withhigh column efficiency and good peak shape characteristics.

- Can be used to separate polar and basic organic compounds.

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