HPLC Tubing Valve Fittings

HPLC Tubing Valve Fittings

Best hplc instrument manufacturers

High Performance HPLC-LC/MS Accessories:

Every lab needs a variety of HPLC accessories. uHPLCs offers special, high-performance polymers and unique materials to meet the needs of your HPLC system. We offer unique products such as biocompatible PEEK tubing. In addition, we offer HPLC fittings for different applications, with plugs, hplc connector, fingertight fittings, adapters, union, tees, crosses, etc. The materials used for fittings are mainly peeking and stainless steel. PEEK is usually the most corrosion-resistant material, suitable for all organic and inorganic solvents, and stable to both acids and bases for most applications. Our HPLC tubing and fittings , frit, and connectors are available in various materials and styles to meet your lab's needs. Contact our support team for more information or to order your fittings today.

 

contact us

 

The shortlist of the characteristics of an ideal connection between components of an LC system:

Leak-free (today, up to 20,000 psi or more);

No unswept volumes (also referred to as voids or dead volumes);

No distortion of connected parts;

The connection can be broken and remade (that is, disconnected and reconnected) many times without changes in performance;

Inexpensive components.

 

Liquid Chromatograph Tubing:

The connection tubing is the basic component for transporting liquid and samples in HPLC. Different materials and specifications can be selected according to the use site and the size of the pressure, mainly stainless steel, PTFE, PEEK, and polyethene or polypropylene tubes. Unsuitable tubing can cause broadening chromatographic peaks or high pressure from blocked liquid flow.

 

- peek tubing hplc:

Polymer tubing has the advantages of being flexible and adapting to the vessel's shape. The parts of the liquid chromatography system where polymer tubing is available are the reservoir to the pump, the detector outlet, and other low-pressure parts such as the injector drain and the vent valve outlet.

 

PEEK (polyether ether ketone) is a high-temperature resistant, high-performance thermoplastic special engineering plastic with good mechanical properties and chemical resistance, abrasion resistance, hydrolysis resistance, etc., can withstand the high pressure of more than 30MPa, more inert than stainless steel pipe, in many cases can replace stainless steel tubing.

 

HPLC Fitting ( hplc tubing connectors )

HPLC Fittings are the connectors of the liquid chromatography system that, with the tubing, connect the components. The connectors should be chemically inert, not leak, and not increase the dead volume of the chromatography system. Improperly matched connectors can create leaks or increase the dead volume.

 

- Low Pressure PEEK Fittings

Low pressure fittings are used to connect plastic (PEEK) tubing, mostly in the peripheral parts of the system, such as the pump's inlet and the detector's outlet. Use PEEK tubing and squeeze it over the low-pressure fitting to seal it.

 

Note: The length of the PEEK tube cut off when using the fitting is important; too short will make the fitting fall off, the dead volume is too large, and too long will not seal tightly and leak. The correct assembly is set on the joint, the PEEK tube of the top of the bottom of the other negative joint hole, and then tighten the nut.

 

- High Pressure Fittings

Stainless steel tubing and stainless steel compression fittings are used for the high-pressure portion of the liquid chromatography system. The assembly of the joint is a stainless steel joint through the tube section, the end of the tube is inserted into the negative joint (a certain fitting or consumable), all the way to the bottom, and the top tightening joint ensures that the outside of the joint is tight against the inside of the negative joint to achieve a seal.

 

Adaptability of common polymeric materials to chemical reagents:

Fluids

PEEK

Acetaldehyde

1

Acetic acid (20%)

1

Acetic acid (80%)

1

Iceacetic acid

1

Acetone

1

Acetonitrile

1

Ammonia(10%)

1

Liquid ammonia

2

Ammonium hydroxide

1

Aromatic hydrocarbons

1

Phenyl

1

Butanol

1

chloroform

1

Cyclohexane

1

Cyclohexanone

1

Diethylamine

1

Diethylether

1

Dioxane

1

Ethanol

1

Ethyl acetate

1

Hexane / Heptane

1

Hydrochloride (100%)

1

Hydrochloride (20%)

1

Isopropanol

1

Isopropyl alcohol

0

Ketones (common)

1

Methanol

1

Dichloromethane

0

Nitrate (100%)

3

Nitrate (20%)

1

Perchlorate

1

Phosphate (100%)

1

Phosphate (20%)

1

NaOH (80%)

1

NaOH (20%)

1

Sulfuric acid (100%)

3

Sulfuric acid (40%)

1

Tetrahydrofuran

1

Toluene

1

Triethylamine

0

Note: 1-Appropriate, no adverse effects; 2-Determined on a case-by-case basis; 3-Inappropriate/not recommended;                  0-No relevant adaptation information

 

Solvent resistance of PEEK materials at high temperatures:

Fluids

Temp / ℃

PEEK Adaptability

Acetic acid

200

2

Liquid ammonia

200

1

Hydrogen sulfide (gas)

200

1

Methane (gas)

200

1

Methanone

200

3

Phosphoric acid (50%)

200

1

NaOH (50%)

200

1

Sulfuric acid (50%)

200

2

Sulfur Dioxide (gas)

200

1

Note: 1-Appropriate, no adverse effects; 2-Determined on a case-by-case basis; 3-Inappropriate/not recommended; 0-No relevant adaptation information

Note: PEEK tubing will swell when containing dimethyl sulfoxide, methylene chloride, and tetrahydrofuran.

 

HPLC Tubing in different parts of the HPLC system:

Part

Type

ID. / mm

Length

Description

Reservoir → Pump

Polytetrafluoroethylene Tubing

1.6

Casual

Oxygen permeable, not for electrochemical detectors

Pump → Injector

Stainless Steel Tubing

1.0

Casual

-

Injector

Stainless steel tubing for dosing ring, polyethylene tubing for venting tube

Depends on sample volume; 0.5

-

-

Injector → HPLC Column

Stainless Steel Tubing

0.2~0.25

Keep it short

-

HPLC Column → Detector

Stainless steel tubing or polyetherketone (PEEK) tubing

0.2~0.25

Keep it short

-

Detector deflate

Polyethylene (propylene) Tubing

0.25

Casual

Use 0.25mm inner diameter 1m long tubing as a restrictor when needed.

Service partner CONTACTs