HPLC Tubing Valve Fittings
Adaptability of common polymeric materials to chemical reagents:
Fluids |
PEEK |
Acetaldehyde |
1 |
Acetic acid (20%) |
1 |
Acetic acid (80%) |
1 |
Iceacetic acid |
1 |
Acetone |
1 |
Acetonitrile |
1 |
Ammonia(10%) |
1 |
Liquid ammonia |
2 |
Ammonium hydroxide |
1 |
Aromatic hydrocarbons |
1 |
Phenyl |
1 |
Butanol |
1 |
chloroform |
1 |
Cyclohexane |
1 |
Cyclohexanone |
1 |
Diethylamine |
1 |
Diethylether |
1 |
Dioxane |
1 |
Ethanol |
1 |
Ethyl acetate |
1 |
Hexane / Heptane |
1 |
Hydrochloride (100%) |
1 |
Hydrochloride (20%) |
1 |
Isopropanol |
1 |
Isopropyl alcohol |
0 |
Ketones (common) |
1 |
Methanol |
1 |
Dichloromethane |
0 |
Nitrate (100%) |
3 |
Nitrate (20%) |
1 |
Perchlorate |
1 |
Phosphate (100%) |
1 |
Phosphate (20%) |
1 |
NaOH (80%) |
1 |
NaOH (20%) |
1 |
Sulfuric acid (100%) |
3 |
Sulfuric acid (40%) |
1 |
Tetrahydrofuran |
1 |
Toluene |
1 |
Triethylamine |
0 |
Note: 1-Appropriate, no adverse effects; 2-Determined on a case-by-case basis; 3-Inappropriate/not recommended; 0-No relevant adaptation information |
Solvent resistance of PEEK materials at high temperatures:
Fluids |
Temp / ℃ |
PEEK Adaptability |
Acetic acid |
200 |
2 |
Liquid ammonia |
200 |
1 |
Hydrogen sulfide (gas) |
200 |
1 |
Methane (gas) |
200 |
1 |
Methanone |
200 |
3 |
Phosphoric acid (50%) |
200 |
1 |
NaOH (50%) |
200 |
1 |
Sulfuric acid (50%) |
200 |
2 |
Sulfur Dioxide (gas) |
200 |
1 |
Note: 1-Appropriate, no adverse effects; 2-Determined on a case-by-case basis; 3-Inappropriate/not recommended; 0-No relevant adaptation information |
Note: PEEK tubing will swell when containing dimethyl sulfoxide, methylene chloride, and tetrahydrofuran.
HPLC Tubing in different parts of the HPLC system:
Part |
Type |
ID. / mm |
Length |
Description |
Reservoir → Pump |
Polytetrafluoroethylene Tubing |
1.6 |
Casual |
Oxygen permeable, not for electrochemical detectors |
Pump → Injector |
Stainless Steel Tubing |
1.0 |
Casual |
- |
Injector |
Stainless steel tubing for dosing ring, polyethylene tubing for venting tube |
Depends on sample volume; 0.5 |
- |
- |
Injector → HPLC Column |
Stainless Steel Tubing |
0.2~0.25 |
Keep it short |
- |
HPLC Column → Detector |
Stainless steel tubing or polyetherketone (PEEK) tubing |
0.2~0.25 |
Keep it short |
- |
Detector deflate |
Polyethylene (propylene) Tubing |
0.25 |
Casual |
Use 0.25mm inner diameter 1m long tubing as a restrictor when needed. |