UHPLCS high performance liquid chromatography preparative hplc column 20*200mm, 10μ, Semi-Prep
uHPLCs high performance liquid chromatography HPLC column Lab 20*200mm, 10μ, Semi-Prep
How to ensure good HPLC column performance and column life
◆ Certifying the reading of the column operating instructions.
◆ Use well-filled columns.
◆ Minimize pressure fluctuations and avoid mechanical and thermal shocks.
◆ Use guard columns and inline filters.
◆ Flush the lc column frequently with a strong solvent.
◆ Filter the sample and mobile phase adequately to avoid impurities and strong retention components. Use a stable stationary phase (C18 is the most stable).
◆ Operate at medium pH (6~8) and use an organic buffer solution.
◆ The column temperature should be less than 40℃.
◆ For columns with a silica gel matrix, the mobile phase’s pH value should be maintained in the range of 3.0~8.0.
◆ Add 200ppm of sodium azide to the aqueous mobile phase and buffer solution.
◆ If the mobile phase contains buffer solution, it should be noted that the transition between water and organic solvent should be 95:5, and the organic solvent should not be less than 5%.
◆ When overnight or in storage, rinse off the salt and buffer solution and store the mobile phase with pure organic solvent (ethylene clear is best).
uHPLCs special services:
1. All columns will be tested before delivery and a column efficiency report will be provided.
2. Column loading service is available.